Hotspot

trnS-GCU-trnG-GCC

LSC · 9391-10088 · 698 bp

Back to Halesia

Pi 0.0101

MarkerSeek score 54.70

Primer available yes

Label trnS-GCU-trnG-GCC

Feature Metadata

label_name: trnS-GCU-trnG-GCC
feature_type: igs
region: LSC
length_bp: 698
variable_sites: 7
pi: 0.0101
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 54.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GCC_p1	AGGAAACGGAAAGAGAGGGA	ACGAATCGCACTTTTACCACT	50.0	42.9	58.0	58.2	820-826 bp	1.0	71.2
trnS-GCU-trnG-GCC_p2	AGGAAACGGAAAGAGAGGGA	ACGAATCGCACTTTTACCAC	50.0	45.0	58.0	56.8	820-826 bp	1.0	71.1
trnS-GCU-trnG-GCC_p3	ATTAGCAATCCGCCGCTTTA	ACGAATCGCACTTTTACCACT	45.0	42.9	58.0	58.2	766-772 bp	1.0	71.1
trnS-GCU-trnG-GCC_p4	ATTAGCAATCCGCCGCTTTA	ACGAATCGCACTTTTACCAC	45.0	45.0	58.0	56.8	766-772 bp	1.0	71.1
trnS-GCU-trnG-GCC_p5	ACGGAAAGAGAGGGATTCGA	ACGAATCGCACTTTTACCACT	50.0	42.9	58.2	58.2	815-821 bp	1.0	70.2