Hotspot

trnK-UUU-rps16

LSC · 4339-5200 · 862 bp

Back to Halesia

Pi 0.0105

MarkerSeek score 56.30

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 862
variable_sites: 9
pi: 0.0105
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CGAATCCCTTGCTTCATCCA	AAGGGCGGAGGTTTTTAAGG	50.0	50.0	58.0	58.1	995-996 bp	1.0	80.2
trnK-UUU-rps16_p2	ACGAATCCCTTGCTTCATCC	AAGGGCGGAGGTTTTTAAGG	50.0	50.0	58.0	58.1	996-997 bp	1.0	80.2
trnK-UUU-rps16_p3	ATGGACGAATCCCTTGCTTC	AAGGGCGGAGGTTTTTAAGG	50.0	50.0	58.0	58.1	1000-1001 bp	1.0	80.2
trnK-UUU-rps16_p4	GGACGAATCCCTTGCTTCAT	AAGGGCGGAGGTTTTTAAGG	50.0	50.0	58.0	58.1	998-999 bp	1.0	80.2
trnK-UUU-rps16_p5	CGAATCCCTTGCTTCATCCA	GGGCGGAGGTTTTTAAGGAA	50.0	50.0	58.0	58.1	993-994 bp	1.0	80.2