Hotspot

trnN-GUU-ndhF

SSC · 110414-112927 · 2514 bp

Back to Halerpestes

Pi 0.261

MarkerSeek score 67.90

Primer available yes

Label trnN-GUU-ndhF

Feature Metadata

label_name: trnN-GUU-ndhF
feature_type: igs
region: SSC
length_bp: 2514
variable_sites: 198
pi: 0.261
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.30
markerseek_score: 67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ndhF_p1	TCAAAGCGAGCCCTTTCATT	GAGGTCCTCGGAGAACTCTT	45.0	55.0	58.1	58.2	2947 bp	0.5	58.0
trnN-GUU-ndhF_p2	GGAACGGGTTACCTACTCCT	GAGGTCCTCGGAGAACTCTT	55.0	55.0	58.1	58.2	2972 bp	0.5	57.6
trnN-GUU-ndhF_p3	AGGATTCGAACCTACGACCA	GAGGTCCTCGGAGAACTCTT	50.0	55.0	58.2	58.2	2887 bp	0.5	57.5
trnN-GUU-ndhF_p4	AAAGCGAGCCCTTTCATTCT	GAGGTCCTCGGAGAACTCTT	45.0	55.0	57.8	58.2	2945 bp	0.5	57.1
trnN-GUU-ndhF_p5	TCAAAGCGAGCCCTTTCATT	AGGTCCTCGGAGAACTCTTT	45.0	50.0	58.1	57.7	2946 bp	0.5	56.8