Hotspot

psbE-petL

LSC · 65510-66697 · 1188 bp

Back to Halerpestes

Pi 0.105

MarkerSeek score 85.90

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1188
variable_sites: 118
pi: 0.105
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 85.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	ACCGAAATAACTAGTTATAGTAGGCA	50.0	34.6	57.9	57.5	1315-1319 bp	1.0	50.8
psbE-petL_p2	ACCAACCCGCAATGAATAGG	ACCGAAATAACTAGTTATAGTAGGCA	50.0	34.6	58.2	57.5	1317-1321 bp	1.0	50.8
psbE-petL_p3	CCGCAATGAATAGGGAAGGT	ACCGAAATAACTAGTTATAGTAGGCA	50.0	34.6	57.7	57.5	1311-1315 bp	1.0	50.8
psbE-petL_p4	ACGAATAACCAACCCGCAAT	ACCGAAATAACTAGTTATAGTAGGCA	45.0	34.6	58.2	57.5	1324-1328 bp	1.0	50.7
psbE-petL_p5	CGAATAACCAACCCGCAATG	ACCGAAATAACTAGTTATAGTAGGCA	50.0	34.6	57.8	57.5	1323-1327 bp	1.0	50.7