Hotspot

trnF-GAA-ndhJ

LSC · 50116-50847 · 732 bp

Back to Halerpestes

Pi 0.0931

MarkerSeek score 84.40

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 732
variable_sites: 62
pi: 0.0931
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 84.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	CGTAAGGATTATATTGCCCCCA	50.0	45.5	58.1	57.7	792-817 bp	1.0	71.4
trnF-GAA-ndhJ_p2	AATCCTCGTGTCACCAGTTC	CGTAAGGATTATATTGCCCCCA	50.0	45.5	57.5	57.7	795-820 bp	1.0	68.1
trnF-GAA-ndhJ_p3	TCGGGATAGCTCAGTTGGTA	CGTAAGGATTATATTGCCCCCA	50.0	45.5	57.6	57.7	830-855 bp	1.0	67.9
trnF-GAA-ndhJ_p4	TGGTAGAGCAGAGGACTGAA	CGTAAGGATTATATTGCCCCCA	50.0	45.5	57.4	57.7	815-840 bp	1.0	66.8
trnF-GAA-ndhJ_p5	TTGGTAGAGCAGAGGACTGA	CGTAAGGATTATATTGCCCCCA	50.0	45.5	57.4	57.7	816-841 bp	1.0	66.8