Hotspot

petN-psbM

LSC · 29551-30773 · 1223 bp

Back to Halerpestes

Pi 0.133

MarkerSeek score 83.70

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1223
variable_sites: 155
pi: 0.133
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 83.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TCTTGCTTGGGCTGCTTTAA	GTCAAGAGTTGTGGAGTGGG	45.0	55.0	58.0	58.1	1882-1961 bp	1.0	91.0
petN-psbM_p2	TCTTGCTTGGGCTGCTTTAA	TCAAGAGTTGTGGAGTGGGA	45.0	50.0	58.0	58.2	1881-1960 bp	1.0	90.4
petN-psbM_p3	TTGACTCTGCGGCATTGATT	GTCAAGAGTTGTGGAGTGGG	45.0	55.0	58.2	58.1	1984-2057 bp	1.0	89.4
petN-psbM_p4	TTTGACTCTGCGGCATTGAT	GTCAAGAGTTGTGGAGTGGG	45.0	55.0	58.2	58.1	1985-2058 bp	1.0	89.4
petN-psbM_p5	ATCCCTTTTTGACTCTGCGG	GTCAAGAGTTGTGGAGTGGG	50.0	55.0	58.2	58.1	2065 bp	0.5	57.7