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trnK-UUU-rps16

LSC · 4332-5095 · 764 bp

Back to Halerpestes

Pi 0.159

MarkerSeek score 75.00

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 764
variable_sites: 77
pi: 0.159
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.63
markerseek_score: 75.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CACATTCGTCTCCACTCAGG	TGAAACGATTCGGGATCACC	55.0	50.0	58.0	58.0	1936 bp	0.5	59.8
trnK-UUU-rps16_p2	TTCTTGGGCCAGCAAATGAT	TGAAACGATTCGGGATCACC	45.0	50.0	58.1	58.0	2163 bp	0.5	59.4
trnK-UUU-rps16_p3	TCAGGATCAGTCGTGGTCTT	TGAAACGATTCGGGATCACC	50.0	50.0	58.1	58.0	1539 bp	0.5	59.2
trnK-UUU-rps16_p4	ATTCACTCGACCCAACCATG	TGAAACGATTCGGGATCACC	50.0	50.0	57.9	58.0	1705 bp	0.5	58.9
trnK-UUU-rps16_p5	CACTCGACCCAACCATGAAT	TGAAACGATTCGGGATCACC	50.0	50.0	57.9	58.0	1702 bp	0.5	58.9