Hotspot

ndhA

SSC · 118690-120943 · 2254 bp

Back to Halenia

Pi 0.0145

MarkerSeek score 55.60

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2254
variable_sites: 32
pi: 0.0145
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TGTTTGAGACAAGTCGTGAA	CACCGGGTTTTATCAATTTGCA	40.0	40.9	55.2	58.1	2372-2410 bp	1.0	43.4
ndhA_p2	TTGTTTGAGACAAGTCGTGA	CACCGGGTTTTATCAATTTGCA	40.0	40.9	55.2	58.1	2373-2411 bp	1.0	43.4
ndhA_p3	TTGTTTGAGACAAGTCGTGAA	CACCGGGTTTTATCAATTTGCA	38.1	40.9	56.0	58.1	2373-2411 bp	1.0	42.5
ndhA_p4	TGTTTGAGACAAGTCGTGAA	ACCGGGTTTTATCAATTTGCA	40.0	38.1	55.2	56.7	2371-2409 bp	1.0	42.5
ndhA_p5	TTGTTTGAGACAAGTCGTGA	ACCGGGTTTTATCAATTTGCA	40.0	38.1	55.2	56.7	2372-2410 bp	1.0	42.5