Hotspot

accD

LSC · 57050-58951 · 1902 bp

Back to Halenia

Pi 0.0101

MarkerSeek score 68.10

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1902
variable_sites: 18
pi: 0.0101
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	ACAAATAAGGGGCAAGAAAACTC	CCCACTCAATGCTCAATTTCA	39.1	42.9	57.5	56.8	2040 bp	0.5	20.0
accD_p2	ACAAATAAGGGGCAAGAAAACTC	ACCCACTCAATGCTCAATTTC	39.1	42.9	57.5	56.8	2041 bp	0.5	20.0
accD_p3	ACAAATAAGGGGCAAGAAAACT	CCCACTCAATGCTCAATTTCA	36.4	42.9	56.4	56.8	2040 bp	0.5	20.0
accD_p4	ACAAATAAGGGGCAAGAAAACT	ACCCACTCAATGCTCAATTTC	36.4	42.9	56.4	56.8	2041 bp	0.5	20.0
accD_p5	CAAATAAGGGGCAAGAAAACTCT	CCCACTCAATGCTCAATTTCA	39.1	42.9	57.3	56.8	2039 bp	0.5	20.0