Hotspot

rpl22-rps19

LSC · 83110-83172 · 63 bp

Back to Halenia

Pi 0.0172

MarkerSeek score 66.30

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: LSC
length_bp: 63
variable_sites: 1
pi: 0.0172
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	GAACGTCCGCGAATTTGATC	GGAAAGGAGCATCTGCCTAT	50.0	50.0	57.9	57.1	291-296 bp	1.0	73.5
rpl22-rps19_p2	ATAGGAACGTCCGCGAATTT	GGAAAGGAGCATCTGCCTAT	45.0	50.0	57.7	57.1	295-300 bp	1.0	71.6
rpl22-rps19_p3	GAACGTCCGCGAATTTGATC	GGAAAGGAGCATCTGCCTATT	50.0	47.6	57.9	57.8	291-296 bp	1.0	71.3
rpl22-rps19_p4	CATAGGAACGTCCGCGAATT	GGAAAGGAGCATCTGCCTAT	50.0	50.0	58.4	57.1	296-301 bp	1.0	70.6
rpl22-rps19_p5	GAACGTCCGCGAATTTGATC	TGGTAGGACACAAATTGGGAG	50.0	47.6	57.9	57.6	254-259 bp	1.0	69.8