Hotspot

psaI-ycf4

LSC · 59319-59766 · 448 bp

Back to Halenia

Pi 0.0156

MarkerSeek score 64.60

Primer available yes

Label psaI-ycf4

Feature Metadata

label_name: psaI-ycf4
feature_type: igs
region: LSC
length_bp: 448
variable_sites: 7
pi: 0.0156
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-ycf4_p1	TAGGCCTAGTATTTCCGGCA	ATAACGGCCCAGCAGAAATT	50.0	45.0	57.9	57.9	599-600 bp	1.0	79.1
psaI-ycf4_p2	CCGGCAATTGCAATAGCTTC	ATAACGGCCCAGCAGAAATT	50.0	45.0	58.2	57.9	585-586 bp	1.0	78.6
psaI-ycf4_p3	TAGGCCTAGTATTTCCGGCA	GATAACGGCCCAGCAGAAAT	50.0	50.0	57.9	58.3	600-601 bp	1.0	77.6
psaI-ycf4_p4	GGCCTAGTATTTCCGGCAAT	ATAACGGCCCAGCAGAAATT	50.0	45.0	57.7	57.9	597-598 bp	1.0	77.6
psaI-ycf4_p5	TTTCCGGCAATTGCAATAGC	ATAACGGCCCAGCAGAAATT	45.0	45.0	57.7	57.9	588-589 bp	1.0	77.5