Hotspot

trnT-GGU-psbD

LSC · 31539-32803 · 1265 bp

Back to Halenia

Pi 0.00952

MarkerSeek score 64.60

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1265
variable_sites: 12
pi: 0.00952
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	GAAACGGTCCCTACGTAACC	55.0	55.0	58.1	58.0	1403-1407 bp	1.0	80.4
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	GAAACGGTCCCTACGTAACC	50.0	55.0	58.1	58.0	1406-1410 bp	1.0	80.2
trnT-GGU-psbD_p3	GTGGTAGAGTAACGCCATGG	GACCAGCCTACAAACACGAA	55.0	50.0	58.1	58.1	1420-1424 bp	1.0	79.4
trnT-GGU-psbD_p4	GTGGTAGAGTAACGCCATGG	AAACACGAAACGGTCCCTAC	55.0	50.0	58.1	58.1	1409-1413 bp	1.0	79.4
trnT-GGU-psbD_p5	GTGGTAGAGTAACGCCATGG	CAAACACGAAACGGTCCCTA	55.0	50.0	58.1	58.1	1410-1414 bp	1.0	79.4