Hotspot

atpH-atpI

LSC · 12813-13775 · 963 bp

Back to Halenia

Pi 0.0198

MarkerSeek score 69.80

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 963
variable_sites: 19
pi: 0.0198
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AAGCAAGTCCTACAGCCAAC	AGGTGAATCTATGGAGGGCC	50.0	55.0	58.1	58.6	1049-1052 bp	1.0	76.5
atpH-atpI_p2	TACCCTCTACAGCTTGACCC	AGGTGAATCTATGGAGGGCC	55.0	55.0	58.1	58.6	1103-1106 bp	1.0	76.2
atpH-atpI_p3	GCGATACCCTCTACAGCTTG	AGGTGAATCTATGGAGGGCC	55.0	55.0	57.9	58.6	1107-1110 bp	1.0	76.1
atpH-atpI_p4	CAGCAGCAATAACAGAAGCG	AGGTGAATCTATGGAGGGCC	50.0	55.0	57.8	58.6	1028-1031 bp	1.0	76.0
atpH-atpI_p5	GAAGCAAGTCCTACAGCCAA	AGGTGAATCTATGGAGGGCC	50.0	55.0	57.8	58.6	1050-1053 bp	1.0	75.9