Hotspot

trnG-UCC-trnR-UCU

LSC · 8930-9110 · 181 bp

Back to Halenia

Pi 0.0331

MarkerSeek score 63.30

Primer available yes

Label trnG-UCC-trnR-UCU

Feature Metadata

label_name: trnG-UCC-trnR-UCU
feature_type: igs
region: LSC
length_bp: 181
variable_sites: 6
pi: 0.0331
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnR-UCU_p1	AGCCTTCCAAGCTAACGATG	GGAAAGAATTTGCGTCCAAT	50.0	40.0	58.0	54.8	307-317 bp	1.0	56.0
trnG-UCC-trnR-UCU_p2	CCTAGCCTTCCAAGCTAACG	GGAAAGAATTTGCGTCCAAT	55.0	40.0	58.1	54.8	310-320 bp	1.0	55.8
trnG-UCC-trnR-UCU_p3	AGCCTTCCAAGCTAACGATG	AGAATTTGCGTCCAATAGGA	50.0	40.0	58.0	54.7	303-313 bp	1.0	55.2
trnG-UCC-trnR-UCU_p4	GACGCTCAAAACGCTGAATC	GGAAAGAATTTGCGTCCAAT	50.0	40.0	58.1	54.8	346-356 bp	1.0	55.1
trnG-UCC-trnR-UCU_p5	CCTAGCCTTCCAAGCTAACG	AGAATTTGCGTCCAATAGGA	55.0	40.0	58.1	54.7	306-316 bp	1.0	55.0