Hotspot

ndhH

SSC · 122571-123761 · 1191 bp

Back to Haemanthus

Pi 0.0194

MarkerSeek score 61.50

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1191
variable_sites: 32
pi: 0.0194
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	CCGCTAGGGCTTGAAGAATT	AGTTGGGTATTCGGGAGTCA	50.0	50.0	58.0	58.1	1565-1574 bp	0.666667	68.3
ndhH_p2	CCGCTAGGGCTTGAAGAATT	TCAGTTGGGTATTCGGGAGT	50.0	50.0	58.0	58.1	1567-1576 bp	0.666667	68.3
ndhH_p3	AATAGATTGAGCAGCAGCCC	AGTTGGGTATTCGGGAGTCA	50.0	50.0	58.0	58.1	1840-1849 bp	0.666667	68.2
ndhH_p4	AATAGATTGAGCAGCAGCCC	TCAGTTGGGTATTCGGGAGT	50.0	50.0	58.0	58.1	1842-1851 bp	0.666667	68.2
ndhH_p5	GTAATTTGGTCCCATCCGCT	AGTTGGGTATTCGGGAGTCA	50.0	50.0	57.9	58.1	1580-1589 bp	0.666667	68.2