Hotspot

ndhD

SSC · 117123-118540 · 1418 bp

Back to Haemanthus

Pi 0.00804

MarkerSeek score 49.40

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1418
variable_sites: 17
pi: 0.00804
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 49.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	AGACTTTGCTTTCTGTCTCA	GACAAAAGCCCGTACTCGAT	40.0	50.0	54.5	58.0	791-1598 bp	1.0	57.3
ndhD_p2	AAGACTTTGCTTTCTGTCTCA	GACAAAAGCCCGTACTCGAT	38.1	50.0	55.4	58.0	792-1599 bp	1.0	56.1
ndhD_p3	AGACTTTGCTTTCTGTCTCA	TTACCGACAAAAGCCCGTAC	40.0	50.0	54.5	58.2	796-1603 bp	1.0	55.7
ndhD_p4	AGACTTTGCTTTCTGTCTCA	TTTTACCGACAAAAGCCCGT	40.0	45.0	54.5	58.3	798-1605 bp	1.0	54.8
ndhD_p5	AAGACTTTGCTTTCTGTCTCA	TTACCGACAAAAGCCCGTAC	38.1	50.0	55.4	58.2	797-1604 bp	1.0	54.5