Hotspot

ndhA

SSC · 121317-122569 · 1253 bp

Back to Haemanthus

Pi 0.142

MarkerSeek score 79.40

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 1253
variable_sites: 175
pi: 0.142
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	GCTCGTATTGTTTGTTGGCC	TGTGAGAGTAGAAGCCCCAA	50.0	50.0	58.0	58.0	286-2234 bp	1.0	91.6
ndhA_p2	GCTCGTATTGTTTGTTGGCC	TGGGGTTTATCAGGACCGAT	50.0	50.0	58.0	58.1	618-2567 bp	1.0	90.9
ndhA_p3	GCTCGTATTGTTTGTTGGCC	GCTACGAGCTTCTGGAATCC	50.0	55.0	58.0	58.1	598-2547 bp	1.0	90.7
ndhA_p4	GCTCGTATTGTTTGTTGGCC	GTGTTTTCCCCTGGAGATGG	50.0	55.0	58.0	58.2	230-2178 bp	1.0	90.5
ndhA_p5	GCTCGTATTGTTTGTTGGCC	GGAATGGGCATTTTTGGTGG	50.0	50.0	58.0	57.9	654-1968 bp	0.666667	69.8