Hotspot

ccsA-ndhD

SSC · 116883-117122 · 240 bp

Back to Haemanthus

Pi 0.0389

MarkerSeek score 67.30

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 240
variable_sites: 14
pi: 0.0389
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	CGAATTCGGCACTTGTAGCT	TCAGTTGACAAAGTACAAGCT	50.0	38.1	58.6	55.4	407-427 bp	1.0	48.7
ccsA-ndhD_p2	ATTCGGCACTTGTAGCTTCT	TCAGTTGACAAAGTACAAGCT	45.0	38.1	57.2	55.4	404-424 bp	1.0	47.7
ccsA-ndhD_p3	ACGAATTCGGCACTTGTAGC	TCAGTTGACAAAGTACAAGCT	50.0	38.1	58.9	55.4	408-428 bp	1.0	46.4
ccsA-ndhD_p4	AATTCGGCACTTGTAGCTTCT	TCAGTTGACAAAGTACAAGCT	42.9	38.1	57.9	55.4	405-425 bp	1.0	45.3
ccsA-ndhD_p5	CGAATTCGGCACTTGTAGCT	ATCAGTTGACAAAGTACAAGCT	50.0	36.4	58.6	55.9	408-428 bp	1.0	45.1