Hotspot

petA-psbJ

LSC · 63978-64977 · 1000 bp

Back to Haemanthus

Pi 0.014

MarkerSeek score 68.10

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1000
variable_sites: 19
pi: 0.014
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCCTTTTGTTCTTCTTGGC	GGTTCATATTCCGGGTTGGG	50.0	55.0	57.8	58.3	1135-1137 bp	1.0	78.0
petA-psbJ_p2	GGCCTTTTGTTCTTCTTGGC	CATATTCCGGGTTGGGTTCA	50.0	50.0	57.8	57.6	1131-1133 bp	1.0	77.1
petA-psbJ_p3	GGCCTTTTGTTCTTCTTGGC	GTTCATATTCCGGGTTGGGT	50.0	50.0	57.8	57.6	1134-1136 bp	1.0	77.1
petA-psbJ_p4	GGCCTTTTGTTCTTCTTGGC	CGGTTCATATTCCGGGTTGG	50.0	55.0	57.8	58.7	1136-1138 bp	1.0	75.0
petA-psbJ_p5	GGCCTTTTGTTCTTCTTGGC	TATTCCGGGTTGGGTTCATC	50.0	50.0	57.8	57.3	1129-1131 bp	1.0	74.8