Hotspot

atpH-atpI

LSC · 14562-15031 · 470 bp

Back to Haemanthus

Pi 0.0128

MarkerSeek score 60.10

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 470
variable_sites: 9
pi: 0.0128
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	ATAGGGGAATCCATGGAGGG	45.0	55.0	58.0	57.9	532-554 bp	1.0	81.7
atpH-atpI_p2	TACCTTGACCAACTCCAGGT	ATAGGGGAATCCATGGAGGG	50.0	55.0	57.9	57.9	588-610 bp	1.0	80.8
atpH-atpI_p3	AATAACGGAAGCGGCAGAAA	ATAGGGGAATCCATGGAGGG	45.0	55.0	58.2	57.9	533-555 bp	1.0	80.5
atpH-atpI_p4	GCAGTACCTTGACCAACTCC	ATAGGGGAATCCATGGAGGG	55.0	55.0	58.2	57.9	592-614 bp	1.0	80.2
atpH-atpI_p5	GCCAATCCAGCAGCAATAAC	ATAGGGGAATCCATGGAGGG	50.0	55.0	57.8	57.9	547-569 bp	1.0	80.2