Hotspot

ycf1

SSC · 124298-129598 · 5301 bp

Back to Habenaria

Pi 0.036

MarkerSeek score 64.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5301
variable_sites: 394
pi: 0.036
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GGAATTTCTTGGGCCCCTTT	TCAAATTGAACAAGCCGCAA	50.0	40.0	58.3	57.1	930-933 bp	1.0	76.9
ycf1_p2	GGAATTTCTTGGGCCCCTTT	TGAACAAGCCGCAAAAGAAA	50.0	40.0	58.3	57.1	924-927 bp	1.0	76.4
ycf1_p3	GGAATTTCTTGGGCCCCTTT	TTGAACAAGCCGCAAAAGAA	50.0	40.0	58.3	57.1	925-928 bp	1.0	76.4
ycf1_p4	TATGGAATTTCTTGGGCCCC	TCAAATTGAACAAGCCGCAA	50.0	40.0	57.5	57.1	933 bp	0.285714	40.7
ycf1_p5	TATGGAATTTCTTGGGCCCC	TGAACAAGCCGCAAAAGAAA	50.0	40.0	57.5	57.1	927 bp	0.285714	40.2