Hotspot

rps16

LSC · 4896-6055 · 1160 bp

Back to Habenaria

Pi 0.0335

MarkerSeek score 59.90

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1160
variable_sites: 83
pi: 0.0335
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	TATTTGTTCGAGACGCCGTT	GAGGTTCGAATCCTTCCGTC	45.0	55.0	57.9	58.1	1890-1958 bp	0.571429	68.2
rps16_p2	AAAGCCGAGTACTCTACCGT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	2255-2336 bp	0.571429	67.7
rps16_p3	AAGCCGAGTACTCTACCGTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	2254-2335 bp	0.571429	67.7
rps16_p4	TATTTGTTCGAGACGCCGTT	AATCCTTCCGTCCCAGATCA	45.0	50.0	57.9	58.1	1882-1888 bp	0.285714	50.0
rps16_p5	AAGCCGAGTACTCTACCGTT	AATCCTTCCGTCCCAGATCA	50.0	50.0	58.2	58.1	2246-2249 bp	0.285714	49.3