Hotspot

rpl32-trnL-UAG

SSC · 114003-114655 · 653 bp

Back to Habenaria

Pi 0.0549

MarkerSeek score 78.30

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 653
variable_sites: 68
pi: 0.0549
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 78.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	CTTTTTGACTGTCCGGTGGA	TGGCGAAACCTTGGGTAAAA	50.0	45.0	58.0	57.9	1191-1301 bp	1.0	89.4
rpl32-trnL-UAG_p2	CTTTTTGACTGTCCGGTGGA	GTCCCGAAGAAATCCAACGA	50.0	50.0	58.0	57.9	1044-1154 bp	0.571429	70.5
rpl32-trnL-UAG_p3	AGTTGAAATGAGCTGGCCAA	TGGCGAAACCTTGGGTAAAA	45.0	45.0	58.0	57.9	1889 bp	0.285714	51.1
rpl32-trnL-UAG_p4	AGTTGAAATGAGCTGGCCAA	GTCCCGAAGAAATCCAACGA	45.0	50.0	58.0	57.9	1742 bp	0.285714	51.0
rpl32-trnL-UAG_p5	AGTTGAAATGAGCTGGCCAA	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	58.0	57.9	1416-1417 bp	0.285714	50.7