Hotspot

ndhF-rpl32

SSC · 113108-113828 · 721 bp

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Pi 0.0492

MarkerSeek score 79.40

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 721
variable_sites: 66
pi: 0.0492
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGAGCGGAAAAAGGGGTATT	TCCACCGGACAGTCAAAAAG	50.0	50.0	57.9	58.0	491-911 bp	1.0	89.8
ndhF-rpl32_p2	ACATAGTAACTGGGAGCGGA	TCCACCGGACAGTCAAAAAG	50.0	50.0	57.8	58.0	503-923 bp	1.0	89.5
ndhF-rpl32_p3	CATTCCCACGAGTTCCCAAA	TCCACCGGACAGTCAAAAAG	50.0	50.0	58.1	58.0	910-1330 bp	1.0	88.5
ndhF-rpl32_p4	AACACATTCCCACGAGTTCC	TCCACCGGACAGTCAAAAAG	50.0	50.0	58.1	58.0	914-1334 bp	1.0	88.3
ndhF-rpl32_p5	TGGCCGAAAAACACATAGCT	TCCACCGGACAGTCAAAAAG	45.0	50.0	58.1	58.0	950-1031 bp	0.714286	73.2