Hotspot

ndhC-trnV-UAC

LSC · 49721-50859 · 1139 bp

Back to Habenaria

Pi 0.0303
MarkerSeek score 74.90
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1139
variable_sites
74
pi
0.0303
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
74.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TGCCAAGATAGGAATAACGCT CCGAGAAAGTCTACGGTTCG 42.9 55.0 57.2 58.1 1191-1322 bp 1.0 73.1
ndhC-trnV-UAC_p2 TGCCAAGATAGGAATAACGCTT CCGAGAAAGTCTACGGTTCG 40.9 55.0 57.8 58.1 1191-1322 bp 1.0 70.6
ndhC-trnV-UAC_p3 ATGCCAAGATAGGAATAACGCT CCGAGAAAGTCTACGGTTCG 40.9 55.0 57.6 58.1 1192-1323 bp 1.0 68.9
ndhC-trnV-UAC_p4 TGCCAAGATAGGAATAACGC CCGAGAAAGTCTACGGTTCG 45.0 55.0 55.6 58.1 1191-1322 bp 1.0 68.7
ndhC-trnV-UAC_p5 TGCCAAGATAGGAATAACGCT GTTCGAGTCCGTATAGCCCT 42.9 55.0 57.2 58.7 1176-1307 bp 1.0 68.5