Hotspot

rps14-psaB

LSC · 36618-36749 · 132 bp

Back to Habenaria

Pi 0.0909

MarkerSeek score 72.50

Primer available yes

Label rps14-psaB

Feature Metadata

label_name: rps14-psaB
feature_type: igs
region: LSC
length_bp: 132
variable_sites: 23
pi: 0.0909
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps14-psaB_p1	CGGTGGGGATTGCAATTTTC	ACTTACGCGGCTTTCTTGAT	50.0	45.0	58.0	57.9	326-348 bp	1.0	88.1
rps14-psaB_p2	GTTCCAATTTCTGGCGCTTC	CCAGTGGCTCTTTCCATTGT	50.0	50.0	58.0	58.1	294-316 bp	1.0	88.0
rps14-psaB_p3	CGGTGGGGATTGCAATTTTC	ATTCACTTACGCGGCTTTCT	50.0	45.0	58.0	57.9	330-352 bp	1.0	88.0
rps14-psaB_p4	CGGTGGGGATTGCAATTTTC	CCAGTGGCTCTTTCCATTGT	50.0	50.0	58.0	58.1	395-417 bp	1.0	86.9
rps14-psaB_p5	CGGTGGGGATTGCAATTTTC	CAATAGGGCCGGGAGATTTC	50.0	55.0	58.0	58.1	952-974 bp	1.0	82.7