Hotspot

trnQ-UUG-psbK

LSC · 6527-6885 · 359 bp

Back to Habenaria

Pi 0.0412

MarkerSeek score 66.50

Primer available yes

Label trnQ-UUG-psbK

Feature Metadata

label_name: trnQ-UUG-psbK
feature_type: igs
region: LSC
length_bp: 359
variable_sites: 30
pi: 0.0412
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-psbK_p1	TCATGTCCTTCAAGTCGCAC	CTTACAGCAGCTTGCCAAAC	50.0	50.0	58.2	57.9	1059-1087 bp	1.0	87.8
trnQ-UUG-psbK_p2	TGCTCGAGCTGCATCATATG	CTTACAGCAGCTTGCCAAAC	50.0	50.0	58.2	57.9	1208-1236 bp	1.0	87.2
trnQ-UUG-psbK_p3	AAGTCGCACGTTGCTTTCTA	CTTACAGCAGCTTGCCAAAC	45.0	50.0	58.1	57.9	1053-1076 bp	0.857143	80.7
trnQ-UUG-psbK_p4	AAGTCGCACGTTGCTTTCTA	ACGCCCCGGATCATTAGATA	45.0	50.0	58.1	58.1	1596-1636 bp	0.428571	60.6
trnQ-UUG-psbK_p5	AAGTCGCACGTTGCTTTCTA	TACGCCCCGGATCATTAGAT	45.0	50.0	58.1	58.1	1597-1637 bp	0.428571	60.6