Hotspot

ycf1

SSC · 124077-129518 · 5442 bp

Back to Gyrotaenia

Pi 0.0103

MarkerSeek score 45.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5442
variable_sites: 56
pi: 0.0103
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 45.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AAAGCGACTGGGGTGTAAAT	GCAGAATACCGTCACCCATT	45.0	50.0	57.4	58.0	260-290 bp	1.0	80.8
ycf1_p2	AAAGCGACTGGGGTGTAAAT	CCGTCACCCATTCTTACCAC	45.0	55.0	57.4	58.3	252-282 bp	1.0	79.0
ycf1_p3	AAAGCGACTGGGGTGTAAAT	GGGAAAGCGGGGAAGAAATA	45.0	50.0	57.4	57.6	206-236 bp	1.0	78.1
ycf1_p4	AAAGCGACTGGGGTGTAAAT	ACCGTCACCCATTCTTACCA	45.0	50.0	57.4	58.6	253-283 bp	1.0	76.0
ycf1_p5	TAAAGCGACTGGGGTGTAAA	GCAGAATACCGTCACCCATT	45.0	50.0	56.8	58.0	261-291 bp	1.0	75.6