Hotspot

clpP

LSC · 70738-72752 · 2015 bp

Back to Gyrotaenia

Pi 0.00847

MarkerSeek score 48.90

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2015
variable_sites: 17
pi: 0.00847
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	CTGAACGAGTCGCACATACA	TACGCAATGGTAGGACGTTG	50.0	50.0	58.0	58.0	2550-2556 bp	1.0	80.7
clpP_p2	TTCACACTCTTTGGTGGCTC	TACGCAATGGTAGGACGTTG	50.0	50.0	58.0	58.0	2979-2990 bp	1.0	80.7
clpP_p3	ATTGGCTGTCGTGTGTTTCT	TACGCAATGGTAGGACGTTG	45.0	50.0	58.0	58.0	2465-2471 bp	1.0	80.6
clpP_p4	CTTTGGTGGCTCTACCCATC	TACGCAATGGTAGGACGTTG	55.0	50.0	58.0	58.0	2971-2982 bp	1.0	80.6
clpP_p5	ATTGGCTGTCGTGTGTTTCT	ATACTCCAACCACCCCATGA	45.0	50.0	58.0	58.0	2934-2941 bp	1.0	80.4