Hotspot

accD

LSC · 57693-59354 · 1662 bp

Back to Gyrotaenia

Pi 0.00431

MarkerSeek score 62.60

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1662
variable_sites: 7
pi: 0.00431
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	ATTCTGATTCGAATCGGCAT	CTACTTGGTCACCCCCAAAC	40.0	55.0	55.3	58.1	1814-1826 bp	1.0	59.7
accD_p2	ATTCTGATTCGAATCGGCAT	TTGGTCACCCCCAAACAAAT	40.0	45.0	55.3	57.8	1810-1822 bp	1.0	59.0
accD_p3	ATTCTGATTCGAATCGGCAT	TAACTACTTGGTCACCCCCA	40.0	50.0	55.3	57.6	1817-1829 bp	1.0	57.1
accD_p4	ATTCTGATTCGAATCGGCAT	ACTACTTGGTCACCCCCAAA	40.0	50.0	55.3	58.5	1815-1827 bp	1.0	56.7
accD_p5	ATTCTGATTCGAATCGGCAT	AACTACTTGGTCACCCCCAA	40.0	50.0	55.3	58.5	1816-1828 bp	1.0	56.6