Hotspot

ndhF-rpl32

SSC · 112338-113478 · 1141 bp

Back to Gyrotaenia

Pi 0.0123
MarkerSeek score 55.30
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1141
variable_sites
14
pi
0.0123
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GGCGAAGATCTTTTGTTGCC TTTTGAGATAGAAGTACGTTTTT 50.0 26.1 58.0 52.4 1289-1291 bp 1.0 41.2
ndhF-rpl32_p2 GGCGAAGATCTTTTGTTGCC TTTTTGAGATAGAAGTACGTTTT 50.0 26.1 58.0 52.4 1290-1292 bp 1.0 41.2
ndhF-rpl32_p3 TGTTGCCGTGGGAAAAAGTA TTTTGAGATAGAAGTACGTTTTT 45.0 26.1 57.9 52.4 1276-1278 bp 1.0 41.2
ndhF-rpl32_p4 TGTTGCCGTGGGAAAAAGTA TTTTTGAGATAGAAGTACGTTTT 45.0 26.1 57.9 52.4 1277-1279 bp 1.0 41.2
ndhF-rpl32_p5 GGCGAAGATCTTTTGTTGCC TTTTTGAGATAGAAGTACGTTTTT 50.0 25.0 58.0 53.2 1290-1292 bp 1.0 41.2