Hotspot

petG-trnW-CCA

LSC · 66839-66961 · 123 bp

Back to Gyrotaenia

Pi 0.0325
MarkerSeek score 71.20
Primer available yes
Label petG-trnW-CCA

Feature Metadata

label_name
petG-trnW-CCA
feature_type
igs
region
LSC
length_bp
123
variable_sites
4
pi
0.0325
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
71.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petG-trnW-CCA_p1 CGTGGTGATCAATTGGACCT AGCGCTCTTAGTTCAGTTCG 50.0 50.0 57.9 58.0 222 bp 1.0 80.4
petG-trnW-CCA_p2 CGTGGTGATCAATTGGACCT AGAACGTGGGTCTCCAAAAC 50.0 50.0 57.9 58.0 200 bp 1.0 80.4
petG-trnW-CCA_p3 ACGTGGTGATCAATTGGACC AGCGCTCTTAGTTCAGTTCG 50.0 50.0 58.2 58.0 223 bp 1.0 79.9
petG-trnW-CCA_p4 ACGTGGTGATCAATTGGACC AGAACGTGGGTCTCCAAAAC 50.0 50.0 58.2 58.0 201 bp 1.0 79.9
petG-trnW-CCA_p5 CGTGGTGATCAATTGGACCT CGCTCTTAGTTCAGTTCGGT 50.0 50.0 57.9 57.7 220 bp 1.0 77.7