Hotspot

psbE-petL

LSC · 65392-66528 · 1137 bp

Back to Gyrotaenia

Pi 0.016

MarkerSeek score 69.10

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1137
variable_sites: 18
pi: 0.016
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AGCTGCTAGTAGAAAACCGA	50.0	45.0	57.9	56.3	1283-1286 bp	1.0	67.9
psbE-petL_p2	AGAACGTTCTCCTGTGCTTC	AGCTGCTAGTAGAAAACCGA	50.0	45.0	57.8	56.3	1206-1209 bp	1.0	67.2
psbE-petL_p3	ACGAATAACCAACCCGCAAT	AGCTGCTAGTAGAAAACCGA	45.0	45.0	58.2	56.3	1292-1295 bp	1.0	66.9
psbE-petL_p4	TGCTGACGAATAACCAACCC	AGCTGCTAGTAGAAAACCGA	50.0	45.0	58.2	56.3	1297-1300 bp	1.0	66.8
psbE-petL_p5	CGAATAACCAACCCGCAATG	AGCTGCTAGTAGAAAACCGA	50.0	45.0	57.8	56.3	1291-1294 bp	1.0	66.8