Hotspot

ndhC-trnV-UAC

LSC · 51226-51783 · 558 bp

Back to Gyrotaenia

Pi 0.0167
MarkerSeek score 70.90
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
558
variable_sites
9
pi
0.0167
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
70.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GGGGCTAAAACTCCGGAAAT AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 669-689 bp 1.0 80.7
ndhC-trnV-UAC_p2 CTGGTCCTTTGCTAATCGGG AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.3 57.8 686-706 bp 1.0 79.1
ndhC-trnV-UAC_p3 CCTTTGCTAATCGGGGCTAA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.7 57.8 681-701 bp 1.0 79.0
ndhC-trnV-UAC_p4 GGGGCTAAAACTCCGGAAAT GAAGGTCTACGGTTCGAGTC 50.0 55.0 57.9 57.5 668-688 bp 1.0 78.0
ndhC-trnV-UAC_p5 TCCTTTGCTAATCGGGGCTA AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.5 57.8 682-702 bp 1.0 77.8