Hotspot

psaA-ycf3

LSC · 42098-42831 · 734 bp

Back to Gyrotaenia

Pi 0.00819

MarkerSeek score 63.60

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 734
variable_sites: 6
pi: 0.00819
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	AGCGCAGAATTGGTTGAAGA	50.0	45.0	57.9	58.1	854-872 bp	1.0	78.9
psaA-ycf3_p2	AACTTCTGGTTCCGACGAAC	AGCGCAGAATTGGTTGAAGA	50.0	45.0	58.1	58.1	804-822 bp	1.0	78.8
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	AAGATCACAAGGCGTTTCGA	50.0	45.0	57.9	57.8	838-856 bp	1.0	78.4
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	AGATCACAAGGCGTTTCGAA	50.0	45.0	57.9	57.8	837-855 bp	1.0	78.4
psaA-ycf3_p5	AACTTCTGGTTCCGACGAAC	AGATCACAAGGCGTTTCGAA	50.0	45.0	58.1	57.8	787-805 bp	1.0	78.3