Hotspot

rps16-trnQ-UUG

LSC · 6236-7007 · 772 bp

Back to Gyrotaenia

Pi 0.0232

MarkerSeek score 69.40

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 772
variable_sites: 17
pi: 0.0232
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	806-845 bp	1.0	83.7
rps16-trnQ-UUG_p2	CGGATCGTGTCCTTCAAGTC	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.4	58.1	821-860 bp	1.0	81.8
rps16-trnQ-UUG_p3	GGATCGTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.4	58.1	820-859 bp	1.0	81.8
rps16-trnQ-UUG_p4	TGGGTGTAAGAATCCACAGC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.5	58.1	840-879 bp	1.0	80.7
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	GGGTTTTGGTCCCGGTATTC	45.0	55.0	58.1	58.5	827-866 bp	1.0	79.9