Hotspot

trnK-UUU-rps16

LSC · 4249-5081 · 833 bp

Back to Gyrotaenia

Pi 0.00866

MarkerSeek score 66.60

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 833
variable_sites: 7
pi: 0.00866
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AGTCGCGGTCTTACAAACTC	ATTTCAAAGAAGGCGGGTGT	50.0	45.0	57.9	58.0	998-1069 bp	1.0	81.9
trnK-UUU-rps16_p2	GTCGCGGTCTTACAAACTCT	ATTTCAAAGAAGGCGGGTGT	50.0	45.0	57.9	58.0	997-1068 bp	1.0	81.9
trnK-UUU-rps16_p3	AAAGCCGAGTACTCTACCGT	ATTTCAAAGAAGGCGGGTGT	50.0	45.0	58.2	58.0	915-986 bp	1.0	81.3
trnK-UUU-rps16_p4	AAGCCGAGTACTCTACCGTT	ATTTCAAAGAAGGCGGGTGT	50.0	45.0	58.2	58.0	914-985 bp	1.0	81.3
trnK-UUU-rps16_p5	CAGTCGCGGTCTTACAAACT	ATTTCAAAGAAGGCGGGTGT	50.0	45.0	58.2	58.0	999-1070 bp	1.0	80.7