Hotspot

ndhD-psaC

SSC · 121835-121947 · 113 bp

Back to Gyrosphragma

Pi 0.0654

MarkerSeek score 86.20

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 113
variable_sites: 7
pi: 0.0654
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 86.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	ACCAAGGAAAATCATTCGTGG	AAACAACTCGAAGCATGGGT	42.9	45.0	56.8	58.0	194-200 bp	1.0	67.4
ndhD-psaC_p2	CCAAGGAAAATCATTCGTGGT	AAACAACTCGAAGCATGGGT	42.9	45.0	56.8	58.0	193-199 bp	1.0	67.4
ndhD-psaC_p3	CCAAGGAAAATCATTCGTGGT	GGCATGAAACAACTCGAAGC	42.9	50.0	56.8	58.0	199-205 bp	1.0	67.2
ndhD-psaC_p4	ACCAAGGAAAATCATTCGTGG	GGCATGAAACAACTCGAAGC	42.9	50.0	56.8	58.0	200-206 bp	1.0	67.1
ndhD-psaC_p5	CCAAGGAAAATCATTCGTGGT	CAGAAGACTGTGTCGGTTGT	42.9	50.0	56.8	57.8	274-280 bp	1.0	64.2