Hotspot

rpl32-trnL-UAG

SSC · 118015-118802 · 788 bp

Back to Gyrosphragma

Pi 0.0245
MarkerSeek score 71.90
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
788
variable_sites
19
pi
0.0245
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
71.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AAAAAGCATATTGGGCAGCG AGCGTGTCTACCGATTTCAC 45.0 50.0 57.7 58.0 895-909 bp 1.0 80.5
rpl32-trnL-UAG_p2 AAAAAGCATATTGGGCAGCG CAGCGTGTCTACCGATTTCA 45.0 50.0 57.7 58.0 896-910 bp 1.0 80.5
rpl32-trnL-UAG_p3 AAAAAGCATATTGGGCAGCG TCCTAAGAGCAGCGTGTCTA 45.0 50.0 57.7 57.9 905-919 bp 1.0 79.5
rpl32-trnL-UAG_p4 GGCAGCGTTGAAAGCTTTTT AGCGTGTCTACCGATTTCAC 45.0 50.0 58.4 58.0 882-896 bp 1.0 79.5
rpl32-trnL-UAG_p5 GGCAGCGTTGAAAGCTTTTT CAGCGTGTCTACCGATTTCA 45.0 50.0 58.4 58.0 883-897 bp 1.0 79.5