Hotspot

rps19-rpl2

IRb · 89172-89248 · 77 bp

Back to Gyrosphragma

Pi 0.0448

MarkerSeek score 68.80

Primer available yes

Label rps19-rpl2

Feature Metadata

label_name: rps19-rpl2
feature_type: igs
region: IRb
length_bp: 77
variable_sites: 3
pi: 0.0448
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl2_p1	TTTTTAGTGAACGTGCCAC	TTGATTCTTCGTCGCCGTAG	42.1	50.0	54.2	58.1	113-123 bp	1.0	46.7
rps19-rpl2_p2	TTTTTAGTGAACGTGCCAC	ATTTGATTCTTCGTCGCCGT	42.1	45.0	54.2	58.3	115-125 bp	1.0	46.0
rps19-rpl2_p3	TTTTTAGTGAACGTGCCAC	TTTGATTCTTCGTCGCCGTA	42.1	45.0	54.2	57.6	114-124 bp	1.0	46.0
rps19-rpl2_p4	TTTTTAGTGAACGTGCCAC	GGGCCCCAATTGGTAGAAAA	42.1	50.0	54.2	58.1	205-215 bp	1.0	45.9
rps19-rpl2_p5	TTTTTAGTGAACGTGCCAC	TTGGGGTTATCCTGCACTTG	42.1	50.0	54.2	57.8	171-181 bp	1.0	45.4