Hotspot

accD-psaI

LSC · 63510-64066 · 557 bp

Back to Gyrosphragma

Pi 0.0259

MarkerSeek score 75.60

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 557
variable_sites: 14
pi: 0.0259
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 75.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AGTTATTGAAGCTTCACGCCT	AAAGAAGCCATTGCCATTGC	42.9	45.0	57.9	57.9	681-808 bp	1.0	74.0
accD-psaI_p2	AGTTATTGAAGCTTCACGCCT	TTGCCATTGCCGGAAATACT	42.9	45.0	57.9	58.2	671-798 bp	1.0	73.7
accD-psaI_p3	AGTTATTGAAGCTTCACGCCT	TGGTACCCGTCGGATCTAAA	42.9	50.0	57.9	57.9	730-857 bp	1.0	73.5
accD-psaI_p4	TTGAAGCTTCACGCCTTTTT	AAAGAAGCCATTGCCATTGC	40.0	45.0	56.8	57.9	676-803 bp	1.0	72.6
accD-psaI_p5	TTGAAGCTTCACGCCTTTTT	TGGTACCCGTCGGATCTAAA	40.0	50.0	56.8	57.9	725-852 bp	1.0	72.0