Hotspot

ndhJ-ndhK

LSC · 53515-53627 · 113 bp

Back to Gyrosphragma

Pi 0.0354

MarkerSeek score 72.90

Primer available yes

Label ndhJ-ndhK

Feature Metadata

label_name: ndhJ-ndhK
feature_type: igs
region: LSC
length_bp: 113
variable_sites: 4
pi: 0.0354
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhJ-ndhK_p1	CTAGCCAAGCAGACAAACGA	AGTTCAGTATCTTCTCACGACT	50.0	40.9	58.2	56.3	174 bp	1.0	50.9
ndhJ-ndhK_p2	TGAACTAGTCGGTGCTTGAC	AGTTCAGTATCTTCTCACGACT	50.0	40.9	57.6	56.3	193 bp	1.0	48.9
ndhJ-ndhK_p3	CTTGACTAGCCAAGCAGACA	AGTTCAGTATCTTCTCACGACT	50.0	40.9	57.5	56.3	179 bp	1.0	48.8
ndhJ-ndhK_p4	ACTAGCCAAGCAGACAAACG	AGTTCAGTATCTTCTCACGACT	50.0	40.9	58.5	56.3	175 bp	1.0	48.6
ndhJ-ndhK_p5	TAGCCAAGCAGACAAACGAC	AGTTCAGTATCTTCTCACGACT	50.0	40.9	58.5	56.3	173 bp	1.0	48.6