Hotspot

psbZ-trnG-GCC

LSC · 39231-39603 · 373 bp

Back to Gyrosphragma

Pi 0.0168

MarkerSeek score 67.50

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 373
variable_sites: 6
pi: 0.0168
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	TTTGCTTCTCCTGACGGTTG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.4	57.8	531-536 bp	1.0	78.5
psbZ-trnG-GCC_p2	ATTTGCTTCTCCTGACGGTT	CGTCTTCTCCTTGGCAAAGA	45.0	50.0	57.4	57.8	532-537 bp	1.0	77.2
psbZ-trnG-GCC_p3	GTATTTGCTTCTCCTGACGGT	CGTCTTCTCCTTGGCAAAGA	47.6	50.0	58.0	57.8	534-539 bp	1.0	73.6
psbZ-trnG-GCC_p4	TGTATTTGCTTCTCCTGACGG	CGTCTTCTCCTTGGCAAAGA	47.6	50.0	58.0	57.8	535-540 bp	1.0	73.5
psbZ-trnG-GCC_p5	CCCGTTGTATTTGCTTCTCC	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	56.8	57.8	540-545 bp	1.0	72.1