Hotspot

trnR-UCU-atpA

LSC · 11108-11447 · 340 bp

Back to Gyrosphragma

Pi 0.024

MarkerSeek score 59.90

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 340
variable_sites: 8
pi: 0.024
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	GGTTCAAATCCTATTGGACGC	GCAACAGAAGGAACGCTTTC	47.6	50.0	57.3	58.0	401-402 bp	1.0	69.1
trnR-UCU-atpA_p2	GGTTCAAATCCTATTGGACGC	AGCAACAGAAGGAACGCTTT	47.6	45.0	57.3	58.3	402-403 bp	1.0	66.9
trnR-UCU-atpA_p3	GGTTCAAATCCTATTGGACGC	CCAGAAGCAGAAGCCCTTTT	47.6	50.0	57.3	58.4	436-437 bp	1.0	66.2
trnR-UCU-atpA_p4	TGGATAGGACAGAGGTCTTCT	GCAACAGAAGGAACGCTTTC	47.6	50.0	56.9	58.0	436-437 bp	1.0	66.0
trnR-UCU-atpA_p5	GGTTCAAATCCTATTGGACGC	AGAAGCAGAAGCCCTTTTGA	47.6	45.0	57.3	57.3	434-435 bp	1.0	63.9