Hotspot

trnS-GCU-trnG-UCC

LSC · 9296-10029 · 734 bp

Back to Gyrosphragma

Pi 0.0141
MarkerSeek score 66.00
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
734
variable_sites
10
pi
0.0141
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA GAATTAAACCGAGGGACCCC 50.0 55.0 58.0 58.0 822-847 bp 1.0 83.0
trnS-GCU-trnG-UCC_p2 CAATCCGACGCTTTAGTCCA GGAATTAAACCGAGGGACCC 50.0 55.0 58.0 58.0 823-848 bp 1.0 83.0
trnS-GCU-trnG-UCC_p3 AATCCGACGCTTTAGTCCAC GAATTAAACCGAGGGACCCC 50.0 55.0 58.0 58.0 821-846 bp 1.0 83.0
trnS-GCU-trnG-UCC_p4 AATCCGACGCTTTAGTCCAC GGAATTAAACCGAGGGACCC 50.0 55.0 58.0 58.0 822-847 bp 1.0 83.0
trnS-GCU-trnG-UCC_p5 GCTCGTACAACGGATTAGCA GAATTAAACCGAGGGACCCC 50.0 55.0 58.1 58.0 840-865 bp 1.0 82.3