Hotspot

trnQ-UUG-psbK

LSC · 7959-8347 · 389 bp

Back to Gyrosphragma

Pi 0.0258

MarkerSeek score 67.00

Primer available yes

Label trnQ-UUG-psbK

Feature Metadata

label_name: trnQ-UUG-psbK
feature_type: igs
region: LSC
length_bp: 389
variable_sites: 10
pi: 0.0258
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-psbK_p1	ATAGGTATTCCCTGGGACGG	TCGGGCAATTTGGCTAAGAA	55.0	45.0	58.0	57.8	549-550 bp	1.0	79.9
trnQ-UUG-psbK_p2	ATAGGTATTCCCTGGGACGG	CTCGGGCAATTTGGCTAAGA	55.0	50.0	58.0	58.2	550-551 bp	1.0	79.5
trnQ-UUG-psbK_p3	GAATACCGGGACCAAAACCC	TCGGGCAATTTGGCTAAGAA	55.0	45.0	58.5	57.8	513-514 bp	1.0	75.9
trnQ-UUG-psbK_p4	GAATACCGGGACCAAAACCC	CTCGGGCAATTTGGCTAAGA	55.0	50.0	58.5	58.2	514-515 bp	1.0	75.6
trnQ-UUG-psbK_p5	GACCAAAACCCGTTGCCTTA	TCGGGCAATTTGGCTAAGAA	50.0	45.0	58.7	57.8	504-505 bp	1.0	74.8