Hotspot

rps16-trnQ-UUG

LSC · 6462-7886 · 1425 bp

Back to Gyrosphragma

Pi 0.0155

MarkerSeek score 60.10

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1425
variable_sites: 18
pi: 0.0155
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	ACCACATCGTTTCAACCGAA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.0	58.1	1218-1479 bp	1.0	84.1
rps16-trnQ-UUG_p2	GCACGTTGCTTTTTACCACA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	57.8	58.1	1232-1493 bp	1.0	82.9
rps16-trnQ-UUG_p3	TCATGTCTGTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	1248-1509 bp	1.0	82.7
rps16-trnQ-UUG_p4	AGTCGCACGTTGCTTTTTAC	GAGGTTCGAATCCTTCCGTC	45.0	55.0	57.6	58.1	1236-1497 bp	1.0	81.4
rps16-trnQ-UUG_p5	CAAGTCGCACGTTGCTTTTT	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.5	58.1	1238-1499 bp	1.0	80.8