Hotspot

trnK-UUU-rps16

LSC · 4464-5343 · 880 bp

Back to Gyrosphragma

Pi 0.0189
MarkerSeek score 61.10
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
880
variable_sites
16
pi
0.0189
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT ACAGAATTTCATTTTAATCAAACG 50.0 25.0 58.2 53.2 924-950 bp 1.0 44.2
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT ACAGAATTTCATTTTAATCAAACG 50.0 25.0 58.2 53.2 923-949 bp 1.0 44.2
trnK-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT CAGAATTTCATTTTAATCAAACGA 50.0 25.0 58.2 52.9 923-949 bp 1.0 44.2
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT CAGAATTTCATTTTAATCAAACGA 50.0 25.0 58.2 52.9 922-948 bp 1.0 44.2
trnK-UUU-rps16_p5 CCGCACTTAAAAGCCGAGTA ACAGAATTTCATTTTAATCAAACG 50.0 25.0 58.3 53.2 933-959 bp 1.0 44.1