Hotspot

ycf1-trnN-GUU

IRa · 149302-149700 · 399 bp

Back to Gyrinops

Pi 0.00621

MarkerSeek score 61.40

Primer available yes

Label ycf1-trnN-GUU

Feature Metadata

label_name: ycf1-trnN-GUU
feature_type: igs
region: IRa
length_bp: 399
variable_sites: 7
pi: 0.00621
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-trnN-GUU_p1	CCCAACGACCGAATTGCTTA	ACGACCAGTCAGTTAACAGC	50.0	50.0	58.6	57.8	532-538 bp	1.0	76.3
ycf1-trnN-GUU_p2	CCCAACGACCGAATTGCTTA	AGGATTCGAACCTACGACCA	50.0	50.0	58.6	58.2	545-551 bp	1.0	76.2
ycf1-trnN-GUU_p3	CCCAACGACCGAATTGCTTA	GGATTCGAACCTACGACCAG	50.0	55.0	58.6	57.8	544-550 bp	1.0	75.8
ycf1-trnN-GUU_p4	CCCAACGACCGAATTGCTTA	CCCCAAGTAGGATTCGAACC	50.0	55.0	58.6	57.7	553-559 bp	1.0	74.8
ycf1-trnN-GUU_p5	CCCAACGACCGAATTGCTTA	TTCGAACCTACGACCAGTCA	50.0	50.0	58.6	58.4	541-547 bp	1.0	74.3