Hotspot

ndhF-rpl32

SSC · 131568-132372 · 805 bp

Back to Gyrinops

Pi 0.0207

MarkerSeek score 64.10

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 805
variable_sites: 41
pi: 0.0207
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AAGGTACGATCCATGAATATT	TGCTCTAGAAGTACGCTTTT	33.3	40.0	52.1	54.4	868-898 bp	1.0	44.9
ndhF-rpl32_p2	AGGTACGATCCATGAATATTT	TGCTCTAGAAGTACGCTTTT	33.3	40.0	52.1	54.4	867-897 bp	1.0	44.9
ndhF-rpl32_p3	AGGTACGATCCATGAATATTT	TTGCTCTAGAAGTACGCTTT	33.3	40.0	52.1	54.4	868-898 bp	1.0	44.9
ndhF-rpl32_p4	AGGTACGATCCATGAATATTT	TTTGCTCTAGAAGTACGCTT	33.3	40.0	52.1	54.4	869-899 bp	1.0	44.9
ndhF-rpl32_p5	AGGTACGATCCATGAATATTT	TTTTGCTCTAGAAGTACGCT	33.3	40.0	52.1	54.4	870-900 bp	1.0	44.9